the effect of different levels of vitamin e in semen extender during the cooling on caspian stallion sperm quality

نویسندگان

ایمان یوسفیان

دانشجوی کارشناسی ارشد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران احمد زارع شحنه

استاد پردیس کشاورزی و منابع طبیعی دانشگاه تهران مهدی ژندی

استادیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران مجید بحرینی

دکتری حرفه ای، مرکز اصلاح نژاد و بهبود تولیدات دامی کشور غزاله ایزدپناه

چکیده

the purpose of this study was to determine the effects of different concentrations of vitamin e (vit. e) in the quality of preserved stallion semen at 5ᵒc for a period of 48 h. semen was collected and most (≥90%) seminal plasma removed by centrifugation at 600 × g. pooled samples divided in four equal parts and diluted with extenders that was supplemented with different concentrations of vit. e [(0 (control, c), 5 and 10 mm)] and dmso positive control group [as vitamin e solvent, (dc)] to a final concentration of 50 × 106 sperm/ml. motility, viability, plasma membrane integrity, total abnormality and lipid peroxidation were determined at 0, 6, 24 and 48h. the results showed that 5mm vitamin e resulted in higher viability and plasma membrane integrity compared to control after 48 h of storage (p0.05). also, 5mm vit. e was decreased lipid peroxidation compared c and dc extenders after 48h of storage (p

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عنوان ژورنال:
علوم دامی ایران

جلد ۴۵، شماره ۱، صفحات ۱-۸

کلمات کلیدی
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